The gene for DNA ligase (EC 220.127.116.11) from thermophilic bacterium Bacillus stearothermophilus NCA1503 has been cloned and the complete nucleotide sequence determined. The ligase gene encodes a protein 670 amino acids in length. The gene was overexpressed in Escherichia coli and the enzyme has been purified to homogeneity. Preliminary characterisation confirms that it is a thermostable, NAD+-dependent DNA ligase. Copyright (C) 1999 Elsevier Science B.V.
|Number of pages||6|
|Journal||Biochimica et Biophysica Acta - Protein Structure and Molecular Enzymology|
|Publication status||Published - 13 Jul 1999|
- Bacillus stearothermophilus
- DNA ligation
- DNA replication
- Thermostable protein